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mammalian expression vector peg bacmam  (Addgene inc)


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    Addgene inc mammalian expression vector peg bacmam
    Mammalian Expression Vector Peg Bacmam, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peg+bacmam+expression+vector/pLPC-NMYC+TRF2+deltaM+(Plasmid+%2316068)/pmc12513491__jciinsight-10-185186-s221-12-9-14
    Average 93 stars, based on 8 article reviews
    mammalian expression vector peg bacmam - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Synthesized:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)
    Article Snippet: The codon- optimized gene encoding the isoform 2 of full- length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI- cells (ATCC # CRL- 3022) as a fusion protein containing an N- terminal Strep- tagII- GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Clone Assay:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)
    Article Snippet: The codon- optimized gene encoding the isoform 2 of full- length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI- cells (ATCC # CRL- 3022) as a fusion protein containing an N- terminal Strep- tagII- GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Modification:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Expressing:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)
    Article Snippet: The codon- optimized gene encoding the isoform 2 of full- length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI- cells (ATCC # CRL- 3022) as a fusion protein containing an N- terminal Strep- tagII- GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Structural Insights into the Activation and Inhibition of the ADAM17-iRhom2 Complex
    Article Snippet: .. The ADAM17 expression vectors were subcloned from pRK5M-ADAM17myc (Addgene: Plasmid #31714) into the pEG BacMam expression vector (Addgene: Plasmid #160451) , . .. The extracellular ADAM17 pro- and metalloprotease domains (residues 1– 477) were cloned into the pLib vector (Addgene: Plasmid #80610), which includes a 6x HisTag at the carboxyl terminus .

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Plasmid Preparation:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)
    Article Snippet: The codon- optimized gene encoding the isoform 2 of full- length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI- cells (ATCC # CRL- 3022) as a fusion protein containing an N- terminal Strep- tagII- GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Structural Insights into the Activation and Inhibition of the ADAM17-iRhom2 Complex
    Article Snippet: .. The ADAM17 expression vectors were subcloned from pRK5M-ADAM17myc (Addgene: Plasmid #31714) into the pEG BacMam expression vector (Addgene: Plasmid #160451) , . .. The extracellular ADAM17 pro- and metalloprotease domains (residues 1– 477) were cloned into the pLib vector (Addgene: Plasmid #80610), which includes a 6x HisTag at the carboxyl terminus .

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Strep-tag:

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Article Title: Transport and InsP8 activation mechanisms of the human inorganic phosphate exporter XPR1
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminal mCerulean and Strep tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. .. XPR1 was expressed in Expi293F cells (Thermo Scientific A14527) using transient transfection.

    Article Title: Transport and InsP 8 gating mechanisms of the human inorganic phosphate exporter XPR1.
    Article Snippet: .. The full-length human XPR1 cDNA (synthesized by Twist Biosciences) was cloned into a modified pEG BacMam expression vector (addgene, 160686) containing a C-terminalmCerulean and Strep-tag (WSHPNFEK) proceeded by a Precision Protease cleavage site (LEVLFQ/GP) to facilitate its removal. ..

    Transduction:

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)
    Article Snippet: The codon- optimized gene encoding the isoform 2 of full- length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI- cells (ATCC # CRL- 3022) as a fusion protein containing an N- terminal Strep- tagII- GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).

    Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)
    Article Snippet: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. .. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP. .. The integrity of the clone was confirmed by Sanger sequencing (Plasmidsaurus, OR).



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